Increasing Ethanol Tolerance and Ethanol Production in an Industrial Fuel Ethanol Saccharomyces cerevisiae Strain
نویسندگان
چکیده
The stress imposed by ethanol to Saccharomyces cerevisiae cells are one of the most challenging limiting factors in industrial fuel production. Consequently, toxicity and tolerance high concentrations has been subject extensive research, allowing identification several genes important for increasing this factor. However, studies were performed with well-characterized laboratory strains, how results obtained these strains work remains unknown. In present work, we have tested three different strategies known increase an fuel–ethanol producing strain: overexpression TRP1 or MSN2 genes, a truncated version gene. Our show that CAT-1 strain tolerates up 14% ethanol, indeed increased its ethanol. When subjected fermentations sugar content cell recycle, simulating conditions used Brazilian distilleries, only gene showed improved fermentation performance, production 16% from 33% total reducing sugars sugarcane molasses. highlight importance testing genetic modifications yeast under order improve S. cerevisiae.
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ژورنال
عنوان ژورنال: Fermentation
سال: 2022
ISSN: ['2311-5637']
DOI: https://doi.org/10.3390/fermentation8100470